Grande Valse Brillante - Ewa Demarczyk - Live (Vinyl, LP, Album)

Presto Recording of the Week, 26th April Building a Library, NovemberFirst Choice. Add CDs to basket. LP The Firebird. Bergen Philharmonic Orchestra, Andrew Litton. The playing on this disc from Andrew Litton and the Bergen Philharmonic is in many ways marvellous, with a good sense of pace throughout much of the ballet. Recorded sound is sensational, especially Add SACD to basket.

View full details Listen to samples. Anna Fedorova piano. Dinu Lipatti piano. As a former pupil of Cortot, it wasn't perhaps surprising that Lipatti always kept a special place in his heart for Chopin. Length 1 track, Related Tags poezja spiewana polish poetry female vocalists ewa demarczyk Add tags View all tags.

Tracklist Sorted by: Running order Running order Most popular. Buy Loading. More Love this track. Play album Buy Loading. External Links Apple Music www. Artist images 37 more. Her songs convey strong emotions.

Search Search. Join others and track this song Grande Valse Brillante - Ewa Demarczyk - Live (Vinyl, find and rediscover music with a Last. Sign Up to Last. Play album. Length Lyrics Add lyrics on Musixmatch. Related Tags poezja spiewana polish poetry Add tags View all tags. Featured On Play album. Live Ewa Demarczyk 10, listeners. Play track. Artist images 37 more.

Her songs convey strong emotions. The genre she repesents is called in Poland sung poetry. Ewa D… read more. The songs are often … read more.

The songs are often based on poems of greatly appreciated "cl… read more. Similar Artists Play all. Trending Tracks 1. Sunday 4 April Monday 5 April Tuesday 6 April Wednesday 7 April Thursday 8 April Friday 9 April Saturday 10 April Sunday 11 April Monday 12 April Tuesday 13 April Wednesday 14 April Thursday 15 April Friday 16 April Saturday 17 April Sunday 18 April Monday 19 April Tuesday 20 April Wednesday 21 April Thursday 22 April Friday 23 April Saturday 24 April Sunday 25 April Monday 26 April Tuesday 27 April Wednesday 28 April Album) 29 April Friday 30 April Saturday 1 May Sunday 2 May Monday 3 May Tuesday 4 May Wednesday 5 May Thursday 6 May Friday 7 May Saturday 8 May Sunday 9 May Monday 10 May It opened the way to new creative projects.

Lio is the mother of 6 children: NubiaEsmeraldaGarance, Grande Valse Brillante - Ewa Demarczyk - Live (Vinyl Leah and Diego She is the sister of singer Helena Noguerra. She was made a Knight of the Order of the Crown in Click here to visit Lio's page on europopmusic. At the start of the Eighties Spanish popmusique was picking up fast. On the forefront of La Movida Madrilena gestated ideological movement in the late seventies in Spain after the fall of the dictatorship of Franco was the bizar persona of Alaska.

Het first combo was called the Pegamoides playing poppy electronic music. The song handles the story of a jealous girl and her husband who is cheating with another.

She follows one night to the address where he commits adultery and, without thinking twice, decided to kill him running over him. Drama all over. The art-work for the single and the album was made by young fashion photographer Javier Vallhonrat who Alaska met during his debut show at the Aele Gallery in Madrid in The single showed two muscled boys wrestling in their underwear, on the album Javier placed Alaska holding a very muscled man.

No wonder rumours started that Alaska was actually a man but all this added to the popularity of the band. Over the years the song has become a sort of gay anthem in Spain.

The fans of Alaska started calling themselves the Alaskitos. In addition to the singles that were released, the album is diversity of musical styles, adjusted to the general synthipop tone of the album. The album went on to sell overcopies in Spain and to more than one and a half million albums worldwide.

It was their biggest success. Click here tot visit Alaska's page on europopmusic. More information on Javier Vallhonrat click here. Raymond van het Groenewoud Belgium. To understand what possessed Belgian singers like Johan Verminnen and Raymond van het Groenewoud to create their own Flemish version of blues we have to take a look to what happened in Belgium in the sixties.

That era started with the violent loss of the colonies, Congo, Rwanda and Burundi. Thus deriving Belgium from valuable resources the country joined the EGKS the pre European Communion conglomerate with its coal and steel industry. In the Flemish movement gained more terrain and the Flemish-Wallonion differences came back on the agenda. This resulted in a new language barrier with Dutch-Flemish on one side and Wallonian-French on the other.

A new language law in fuelled the so called linguistic wars that still go on today. And last but not least the violence of WW1 and 2 left deep, deep scars in the Flanders country. Due to this most of the post-war Marshall help went to Flanders Doing so a significant percentage of the foreign capital particularly from the United Statescoming into Belgium to support new industries was being invested in Flanders.

In contrast, Wallony's coal mines and time-worn steel plants and factories were in crisis. The region had lost thousands of jobs and much investment capital. A new Dutch-speaking, upwardly mobile "populist bourgeoisie" was not only becoming visible and vocal in Flemish movements but also in both the local and national policy. Only in was an official Dutch version of the Constitution accepted. To know this is to understand why Verminnen and friends were so eager to create their own version of the blues.

And in the Flemish language. They mirror the Flemish unemployed workman eager for love, sex, beer or just some recognition but stuck in old traditions. Searching for a meaning to his life. Unlike Verminnen who is more romantic by nature Van het Groenewoud stays close to the themes of the blues but with a unique Flemish signature. Commercially this was a maybe not the wisest choice since singing pop and rock in your own language was not considered very cool in those days.

In Album) eighties he is able to make a switch to more general themes and so wins a steady fanbase in Belgium and Holland. He refers to himself as a musician but Album) a poet, philosopher and clown. And that he is. Click here to visit Raymond's page on europopmusic. If you're searching for a good introduction to Van Het Groenewoud's work; Dutch collector Vic van der Reijt just released a three-disc compilation celebrating Raymond's anniversary.

Under the title 'Omdat ik van je hou' Because i love you it is split in a section protest-songs, comedy-tracks and his bittersweet songs. Venditti made name for himself in the s with the social themes woven into his songs. With the LP Lilly: the yearning title-track was a strong accusation, this time against drugs, but it met an outstanding success anyway. Anyway, some events in like the public booing of his friend De Gregori by politicized fans during a show forced Antonello to rethink his way of being a public personality.

The title track is one of the best-known songs Venditti, a song attempting to contemplate on the lives of contemporaries of the singer, who had lived through the '68 and the years of commitment and that they were preparing to face the years of reflux, and it is also an educational response to the charges against him for having "sold out to the market".

The title refers to the zodiacal sign of the singer who was born on 8 March As they unite all their fellow youth in a single matrix, however fictitious because it is not an astrological sign that can guarantee the concept of "love and unity After the De Gregori incident Venditti decided to dedicate two songs to his musical friend.

Especially the last one caused a storm with the Italian feminist movement. Probably inspired by a real person, the text provides no hints to be able to identify this with certainty. The album cover, by Mario Convertino, is white, designed with two fish, one blue and one orange in the first edition there were also marked in relief all twelve signs of the zodiac.

The album was released on the singer's birthday, March 8, and would become against Venditti's intention the soundtrack of a dark period in Italian history only 8 days later, on March 16 Aldo Moro was kidnapped by the Red Brigades. After the somewhat disappointing sales of the previous album, Venditti was back to the top with the single of the title song. Click here tot visit Antonello's page on europopmusic.

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Ferry Tayle - Lozza (File)

Start the wiki. Don't want to see ads? Upgrade Now. Javascript is required to view shouts on this page. Go directly to shout page. View full artist profile. View all similar artists. View all trending tracks. Submit Lyrics. Library Artists Billboard Contributes. Lozza - Ferry Ferry Tayle - Lozza (File). Print it! Download Font Style Embed.

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Aylin [Radio Edit] Standerwick Ferry Tayle - Lozza (File) Dreamstate Radio Edit Friday 20 August Saturday 21 August Sunday 22 August Monday 23 August Tuesday 24 August Wednesday 25 August Thursday 26 August Friday 27 August Saturday 28 August Sunday 29 August Monday 30 August Tuesday 31 August Wednesday 1 September Thursday 2 September Friday 3 September Saturday 4 September Sunday 5 September Monday 6 September Tuesday 7 September Wednesday 8 September Thursday 9 September Friday 10 September Saturday 11 September Sunday 12 September Monday 13 September Tuesday 14 September Wednesday 15 September Thursday 16 September Friday 17 September Saturday 18 September Sunday 19 September Monday 20 September Tuesday 21 September Wednesday 22 September Thursday 23 September Ferry Tayle - Lozza (File) Friday 24 September Saturday 25 September Sunday 26 September Monday 27 September Tuesday 28 September Wednesday 29 September Thursday 30 September Friday 1 October Saturday 2 October Monday 26 July Tuesday 27 July Wednesday 28 July Thursday 29 July Friday 30 July Saturday 31 July Sunday 1 August Monday 2 August Tuesday 3 August Wednesday 4 August Thursday 5 August Friday 6 August Saturday 7 August Ferry Tayle - Lozza (File) 8 August Monday 9 August Tuesday 10 August Wednesday 11 August Thursday 12 August Friday 13 August Saturday 14 August Sunday 15 August Monday 16 August Tuesday 17 August Wednesday 18 August Thursday 19 August Friday 20 August Saturday 21 August Sunday 22 August Monday 23 August Tuesday 24 August Wednesday 25 August Thursday 26 August Friday 27 August Saturday 28 August Sunday 29 August Monday 30 August Tuesday 31 August Wednesday 1 September Thursday 2 September Friday 3 September Saturday 4 September Ferry Tayle - Lozza (File) Sunday 5 September Monday 6 September Tuesday 7 September

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La fontana (da « Bestie ») - Various - Primo Novecento (Vinyl, LP)

The record cover fiasco. Speculation about how this kind of quality control gaffe could occur was that the photos were all in an alphabetically arranged set of folders, and when reaching in to the "Dave Clark Five" folder, a hand accidentally reached into the folder behind it, which was "Dave Dee. But the really funny part of the situation was that nobody noticed the error until LP) album was in the stores!

By then, they just figured, "Ignore it. No one will notice. The other Fontana band that debuted with a 1 single in was the New Vaudeville Band, whose throwback to Rudy Valee and the megaphone days, "Winchester Cathedral" [Fontana ], caught the record buyers' fancy late in the year.

Surprisingly, the record became an international hit, and he needed a group to go on the road to perform. Alas, a common tale, but a group was hired as "The New Vaudeville Band" and toured for a couple of years.

Three more singles in the same vein followed, including "Peek-A-Boo" [Fontana72], "Finchley Central" [Fontana], "Green Street Green" [Fontana ], and "Bonnie and Clyde" [Fontana], after which the megaphone revival was put to rest. In retrospect, was the best year for Fontana, with two 1 hits and a 2 hit LP) addition to several other chart records.

By contrast to were pretty much all downhill. Releases by the new British band, The Herd, featuring the young guitarist Peter Frampton, were ignored, which was proving the rule for all Fontana's artists.

Frampton wasn't really ignorable. In the s he had some of the best selling LPs of all time as well as numerous hits; in early he's constantly on TV and radio hawking Geico Insurance.

All to no avail, chartwise. Other than the early hit "Love Is All Around" by the Troggs, there were only two more records of note before Mercury finally pulled the plug on the La fontana (da « Bestie ») - Various - Primo Novecento (Vinyl in early By the time the group was ready to put out a followup and an album in earlyFontana was in the process of going under, and the album and followup single "I've Gotta Make You Love Me" [Mercury ] were released on the parent Mercury label.

The song featured a simulated sex session, with Birkin panting and whispering "I Love You" while deep-voiced Gainsbourg replied with phrases amounting to "Me, neither. Birkin was an actress, having appeared in, among other films, the Yardbirds' cult classic Blow Up. She was at one time married to British band leader John Barry, but in she married French songwriter Lucien "Serge" Gainsbourg, who had been successfully writing French rock songs for a few years.

Many of these, like "The Lollipops," had a double meaning, but when he recorded "Je T'Aime" with Bridget Bardot, the song was so hot and blatantly sexual that Bardot hesitated to agree to its release. He recorded a second version with his wife, and it was this version that became an international phenomenon, if not an international scandal. With a song like that, nothing could really follow it, so their US career, as well as the label itself, closed down.

Early singles used the Smash numbering sequence, as indicated by the "S-" prefix on the label at far left. About February,a new Fontana singles series was established, with an "F-" prefix. Deejay versions were white with black print. The same design was used for the label's entire life from Reissue 45s used a different label design, as shown at near left. The album labels throughout Fontana's existence were light blue with black print.

Mono labels, as shown on the far left, had "A Custom High Fidelity La fontana (da « Bestie ») - Various - Primo Novecento (Vinyl to the right of the center hole, while the stereo versions shown near left had "A Stereophonic High Fidelity Recording. There were, however, two known Fontana inner sleeves. Bythey had been used up and Fontana reverted to plain sleeves. We would appreciate any additions or corrections to this discography. Just send them to us via e-mail. L'Osservatore Mejor lector ebook Romano.

Archivado desde el original el 28 de septiembre de Consultado el 11 de agosto de The Guardian London. Archivado desde el original el 13 de febrero de Consultado el 8 de septiembre de Implemented both as Stylistic Set n1 ss01 and Stylistic Alternate salt to maximize compatibility between applications.

Implemented both LP) Stylistic Set n2 ss02 and Stylistic Alternate salt to maximize compatibility between applications. Implemented both as Stylistic Set n3 ss03 and Stylistic Alternate salt to maximize compatibility between applications. Implemented both as Stylistic Set n4 ss04 and Stylistic Alternate salt to maximize compatibility between applications.

Implemented both as Stylistic Set n5 ss05 and Stylistic Alternate salt to maximize compatibility between applications.

Figures numbers, monetary and math symbols of same width always align, in spite of their weight. Works with tabular and proportional figures, numerators, denominators and superiors.

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Stay - Bonnie Bianco - Best Of Bonnie Bianco (Vinyl, LP)

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Furthermore, a thin cam timing oil control valve assembly was installed on the front surface side of the timing chain cover to make the variable valve timing mechanism more compact. The cam timing oil control valve assembly operated according to signals from the ECM, controlling the position of the spool valve and supplying engine oil to the advance hydraulic chamber or retard hydraulic chamber of the LP) timing gear assembly.

To alter cam timing, the spool valve would be activated by the cam timing oil control valve assembly via a signal from the ECM and move to either the right to advance timing Stay - Bonnie Bianco - Best Of Bonnie Bianco (Vinyl the left to retard timing. Ghost in the Machine Remastered The Police. Sting Essentials Apple Music Pop.

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Folarin II Wale. More to Explore. Browse by Category. Apple Music on Apple News. Top Charts. The variable valve lift system only changed the lift of one of the intake valves LP) each cylinder since the other intake valve always provided high lift. The operation of the variable valve lift system may be summarised as follows: At engine speeds below rpm, valve lift for one of the intake valves was reduced to increase intake air speed and to create a swirl effect within the combustion chamber since the lift of the two intake valves differed.

As a result of the faster intake air speed and better mixture formation, a higher torque output was achieved; At engine speeds between rpm and with high loads, valve lift for the variable intake valve would be increased to reduce intake resistance for greater power; and, At engine speeds above rpm, high valve lift would be provided regardless of load.

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Najtrudniej - Onar i O$ka - $uperelak$ (Cassette, Album)

Znaci samo cinc kao za mikrofon i slusalice, je l'? Ako trazis neko kvalitetnije resenje razmisli o zameni zvucne kartice barem neki Audigy SE ili slicno razlika je primetna. Sto se tice spajanja zavisi Najtrudniej - Onar i O$ka - $uperelak$ (Cassette toga kakav ti Najtrudniej - Onar i O$ka - $uperelak$ (Cassette izlaz na kasetofonu.

Ako imas standardni izlaz za slusalice na istom onda kupi kabl sa 3,5mm bananicom na jednom karaju i istom takvom na drugom. Ako ti je izlaz na kasetofonu sa "velikim" izlazom onda kupi kabl sa 6,25mm jack-om sa jedne strane i 3,5mm sa druge. Najeftinije jednostavno znaci nekvalitetno, mada uvek moze da se uradi dosta na poboljsanju kvaliteta.

Za prebacivanje ti je potreban bilo kakav deck, da li on bio deo nekog kasetofona ili odvojeno pa cak i wallkman moze da zavrsi posao. Ali najbolje da to bude neki uredjaj koji ima Line Out, sto znaci da je to izlaz na koji ne utice eq, volumen, znaci cist izlaz, plus bi bilo veoma nego pozeljno da deck ima Dolby NR bilo kakav vid. Ukoliko nemas uredja sa Line Out-om i moras da ukljucis na izlazu za slusalice, postoji mogucnost da rezultujuci snimak ima clipping i distorzije, u takvim slucajevima mozes donekle da emuliras line out tako sto ces eq da ugasis ili ga postavis na sredinu, a volumen da pojacas ili smanjis dok ne dobijes odgvarajuce.

Indeed, this album is the very definition of what we have aimed to do as a label over the past eight years, and I am so thankful to be releasing a work of such Najtrudniej - Onar i O$ka - $uperelak$ (Cassette beauty as to define who we are and what we stand for.

So here we have a work by ghost and tape, an album pure, forceful, mystifying, and beautiful. Like Liked by 1 person. You are commenting using your WordPress. Idite na Album) — Album). Odaberite karticu Audio Options. Objavljeno od Alexxandar. Ostavite odgovor Odustani od odgovora Unesite svoj komentar ovde Poruka 1. NICS Iskusan. Poruka 5. Prijavite se ili registrujte da biste poslali poruku.

Registrujte se. Blokirali ste reklame. Sign in with Facebook. Facebook Twitter Instagram Youtube. Sign in to follow this Followers 2. Reply to Album) topic Start new topic.

Recommended Posts. Posted July 24, Drugari, Kopao sam ali nigde ne mogu da nadjem odgovor na svoje pitanje. Share this post Link to post Share on other sites. Nisam kraj racunara da ti napisem ali ako znas engleski evo ti link. Tako sam ja pre rezao.

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Take Me - Kiss - Alive In San Francisco (CD)

At Pacific, we are sensitive to the challenges students have faced this year and treat every admission application with care. Our community is among the most diverse in the nation. Our students are bright, determined, and above all else, Take Me - Kiss - Alive In San Francisco (CD) it together. From the classroom to co-curriculars, our students create connections that reach far beyond their time on campus. Located in Northern California, University of the Pacific offers a gateway to some of the fastest growing economic and cultural centers in the world.

Pacific and the surrounding area are on the cutting edge of science and math-based disciplines, health, the arts, and business innovation. With our individualized approach, students receive personal attention that supports their growth and academic ambitions.

Our campuses in Stockton, Sacramento, and San Francisco offer students the unique opportunities each city provides. With our wide range of distinct and experiential programs, at each of our campuses you'll find expansive opportunities to connect to California's and the country's fastest growing ecosystem of industrial, technological, social, cultural, and economic growth.

If the family is all available to sit down and watch a flick, you might want to choose a G rated movie. Movies can also be searched by their rating. You can be family Take Me - Kiss - Alive In San Francisco (CD) with your Take Me - Kiss - Alive In San Francisco (CD) or you can pick one strictly for adults when the time is right.

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After 27 years of marriage Isabel and Willie go their separate ways. Ripper is published simultaneously in English and Spanish. Isabel begins a yearlong sabbatical in January. Nobody quite believes it. Island Beneath the Sea published in Spanish. The Sum of Our Days published in Spanish. Zorro published in Spanish. Isabel inducted into the American Academy of Arts and Letters. Isabel becomes an American citizen.

Aphrodite published in Spanish. Paula published in Spanish, German, and Dutch. Of Love and Shadows made into a movie; Antonio Banderas stars.

The Infinite Plan published in English. Witcher Wiki. The Boys Wiki. Avatar Wiki. Titans Wiki. Sex Education Wiki. SpongeBob Wiki. Eric Goldman Sep Finding the Perfect Horror Location. Showrunner David S Goyer and Take Me - Kiss - Alive In San Francisco (CD) Jared Harris and Lee Pace talk the sci-fi's complex characters and the rise in interest in heroes over villains. Max Covill Sep Eric Goldman Sep 6. How Team 'What If? The latest trends, delivered straight to your inbox.

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Vargsång - Ultima Thule (2) - Vikingabalk (Vinyl, LP, Album)

Jake Dieffenbach stops by to discuss his latest album and his life as a deaf heavy metal singer. Explore music. Get fresh music recommendations delivered to your inbox every Friday. Jake Vargsång - Ultima Thule (2) - Vikingabalk (Vinyl. Mushroom Head.

Norman Truscott. Purchasable with gift card. A superb collection of rare early tracks from electronic pioneers Tangerine Dream! Moder Jord svenska. My Land engelska. My Love engelska. Ny nord svenska. Om Skallagrims Son svenska.

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Stolt och Stark svenska. Stolt Svensk svenska. LP Arv, Svealand svenska. The band seems to have had a mail-order distribution deal with the record company Ragnarock at some stage.

Between and the band played at about 30 skinhead concerts. In response to the allegations of racism, the band has issued a statement on their website making clear they do not consider themselves racist, nor are they neo-nazis. In their own words. If we Swedes Album) not allowed to show esteem and pride about our own country without being called racists, this makes [it] much more difficult to show others respect for their own particular identity and singular characteristics.

During a concert in some members of the audience made Hitler salutes. The band stopped playing to make clear that they did not want to be seen playing for Nazis. From Wikipedia, the free encyclopedia. Not to be confused with Ultima Thule Estonian band. Music and Arts in Action. Use short dishwashing programs and low temperatures. It is best to hand wash delicate glasses, pitchers and jugs.

Do not stack warm Album) immediately after washing as they may get stuck and break. When serving hot drinks, we recommend glasses made from special heat resistant glass. Iittala glass is not oven safe nor Album) we recommend you put them in the microwave.

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Je Me Suis Fait Tout Petit - The Gypsy Swing Project - Paris - Dubai (CD)

Je me suis fais tout petit Remastered. Georges Brassens. Ascolta ora. Per approfittare dei vantaggi offerti da Prime Music, accedi alla Libreria musicale e trasferisci il tuo account su Amazon.

Aggiungi alla libreria musicale. Ascolta un estratto del brano. Recensioni clienti. Come vengono calcolate le valutazioni? Per calcolare la valutazione complessiva in stelle e la ripartizione percentuale per stella, non usiamo una media semplice. Il nostro sistema Je Me Suis Fait Tout Petit - The Gypsy Swing Project - Paris - Dubai (CD) elementi quali la recente recensione e se il revisore ha acquistato l'articolo su Amazon. Fermer Privacy Overview This website uses cookies to improve your experience Je Me Suis Fait Tout Petit - The Gypsy Swing Project - Paris - Dubai (CD) you navigate through the website.

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We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. I was surprised to meet a French friend living in Norway telling me that the people of his band never saw each other except in public places bars, restaurants or gigs! You play in Je Me Suis Fait Tout Petit - The Gypsy Swing Project - Paris - Dubai (CD) same band or you live Je Me Suis Fait Tout Petit - The Gypsy Swing Project - Paris - Dubai (CD) a city with only 10 people playing jazz manouche and no one ever organizes a jam at home?

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Online Dating Guide. No matter who you ask, you will get the same answer: dating nowadays is hard. For single expats in Germany, dating is even harder. Online Dating. In a perfect world, you and your soulmate would bump into each other on the streets of Germany, lock eyes, and fall madly in love the next second. Dating Profile. In bacteriathis overlap may be involved in the regulation of gene transcription, [35] while in viruses, overlapping genes increase the amount of information that can be encoded within the small viral genome.

With DNA in its "relaxed" state, a strand usually circles the axis of the double helix once every If they are twisted in the opposite direction, this is negative supercoiling, and the bases come apart more easily.

In nature, most DNA has slight negative supercoiling that is introduced by enzymes called topoisomerases. Although the B-DNA form is most common under the conditions found in cells, [44] it is not a well-defined conformation but a family of related DNA conformations [45] that occur at the high hydration levels present in cells.

Their corresponding X-ray diffraction and scattering patterns are characteristic of molecular paracrystals with a significant degree of disorder. Here, the strands turn about the helical axis in a left-handed spiral, the opposite of the more common B form. For many years, exobiologists have proposed the existence of a shadow biospherea postulated microbial biosphere of Earth that uses radically different biochemical and molecular processes than currently known life.

One of the proposals was the existence of lifeforms that use arsenic instead of phosphorus in DNA. A report in of the possibility in the bacterium GFAJ-1was announced, [52] [53] though the research was disputed, [53] [54] and evidence suggests the bacterium actively prevents the incorporation of arsenic into the DNA backbone and other biomolecules. At the ends of the linear chromosomes are specialized regions of DNA called telomeres.

These guanine-rich sequences may stabilize chromosome ends by forming structures of stacked sets of four-base units, rather than the usual base pairs found in other DNA molecules.

Here, four guanine bases, known as a guanine tetradform a flat plate. These flat four-base units then stack on top of each other to form a stable G-quadruplex structure. In addition to these stacked structures, telomeres also form large loop structures called telomere loops, or T-loops.

Here, the single-stranded DNA curls around in a long circle stabilized by telomere-binding proteins. This triple-stranded structure is called a displacement loop or D-loop. In DNA, fraying occurs when non-complementary regions exist at the end of an otherwise complementary double-strand of DNA. However, branched DNA can occur if a third strand of DNA is introduced and contains adjoining regions able to hybridize with the frayed regions of the pre-existing double-strand.

Although the simplest example of branched DNA involves only three strands of DNA, complexes involving additional strands and multiple branches are also possible. Several artificial nucleobases have been synthesized, and successfully incorporated in the eight-base DNA analogue named Hachimoji DNA.

Their existence could be seen as an indication that there is nothing special about the four natural nucleobases that evolved on Earth. For this purpose it has to fold into a structure. It has been shown that to allow to create all possible structures at least four bases are required for the corresponding RNA[66] while a higher number is also possible but this would be against the natural Principle of least effort.

The expression of genes is influenced by how the DNA is packaged in chromosomes, in a structure called chromatin. Base modifications can be involved in packaging, with regions that have low or no gene expression usually containing high levels of methylation of cytosine bases. DNA packaging and its influence on gene expression can also occur by covalent modifications of the histone protein core around which DNA is wrapped in the chromatin structure or else by remodeling carried out by chromatin remodeling complexes see Chromatin remodeling.

There is, further, crosstalk between DNA methylation and histone modification, so they can coordinately affect chromatin and gene expression. For one example, cytosine methylation produces 5-methylcytosinewhich is important for X-inactivation of chromosomes. Mutagens include oxidizing agentsalkylating agents and also high-energy electromagnetic radiation such as ultraviolet light and X-rays. The type of DNA damage produced depends on the type of mutagen. For example, UV light can damage DNA by producing thymine dimerswhich are cross-links between pyrimidine bases.

Because of inherent limits in the DNA repair mechanisms, if humans lived long enough, they would all eventually develop cancer. Although most of these damages are repaired, in any cell some DNA damage may remain despite the action of repair processes. These remaining DNA damages accumulate with age in mammalian postmitotic tissues. This accumulation appears to be an important underlying cause of aging. Many mutagens fit into the space between two adjacent base pairs, this is called intercalation.

Most intercalators are aromatic and planar molecules; examples include ethidium bromideacridinesdaunomycinand doxorubicin. For an intercalator to fit between base pairs, the bases must separate, distorting the DNA strands by unwinding of the double helix. This inhibits both transcription and DNA replication, causing toxicity and mutations.

DNA usually occurs as linear chromosomes in eukaryotesand circular chromosomes in prokaryotes. The set of chromosomes in a cell makes up its genome ; the human genome has approximately 3 billion base pairs of DNA arranged into 46 chromosomes.

Transmission of genetic information in genes is achieved via complementary base pairing. Usually, this RNA copy is then used to make a matching protein sequence in a process called translationwhich depends on the same interaction between RNA nucleotides.

In alternative fashion, a cell may simply copy its genetic information in a process called DNA replication. The details of these functions are covered in other articles; here the focus is on the interactions between DNA and other molecules that mediate the function of the genome. Genomic DNA is tightly and orderly packed in the process called DNA condensationto fit the small available volumes of the Lifeforms (Path 1) - The Future Sound Of London - Cascade / Lifeforms (CD).

In eukaryotes, DNA is located in the cell nucleuswith small amounts in mitochondria and chloroplasts. In prokaryotes, the DNA is held within an irregularly shaped body in the cytoplasm called the nucleoid. A gene is a unit of heredity and is a region of DNA that influences a particular characteristic in an organism.

Genes contain an open reading frame that can be transcribed, and regulatory sequences such as promoters and enhancerswhich control transcription Lifeforms (Path 1) - The Future Sound Of London - Cascade / Lifeforms (CD) the open reading frame. In many speciesonly a small fraction of the total sequence of the genome encodes protein. For example, only about 1.

Some noncoding DNA sequences play structural roles in chromosomes. Telomeres and centromeres typically contain few genes but are important for the function and stability of chromosomes. A gene is a sequence of DNA that contains genetic information and can influence the phenotype of an organism. Within a gene, the sequence of bases along a DNA strand defines a messenger RNA sequence, which then defines one or more protein sequences.

The relationship between the nucleotide sequences of genes and the amino-acid sequences of proteins is determined by the rules of translationknown collectively as the genetic code.

The genetic code consists of three-letter 'words' called codons formed from a sequence of three nucleotides e. Since there are 4 bases in 3-letter combinations, there are 64 possible codons 4 3 combinations. These encode the twenty standard amino acidsgiving most amino acids more than one possible codon. Cell division is essential for an organism to grow, but, when a cell divides, it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent.

Here, the two strands are separated and then each strand's complementary DNA sequence is recreated by an enzyme called DNA polymerase. This enzyme makes the complementary strand by finding the correct base through complementary base pairing and bonding it onto the original strand.

It may act as a recognition factor to regulate the attachment and dispersal of specific cell types in the biofilm; [] it may contribute to biofilm formation; [] and it may contribute to the biofilm's physical strength and resistance to biological stress. Cell-free fetal DNA is found in the blood of the mother, and can be sequenced to determine a great deal of information about the developing fetus.

Under the name of environmental DNA eDNA has seen increased use in the natural sciences as a survey tool for ecologymonitoring the movements and presence of species in water, air, or on land, and assessing an area's biodiversity. All the functions of DNA depend on interactions with proteins.

These protein interactions can be non-specific, or the protein can bind specifically to a single DNA sequence. Within chromosomes, DNA is held in complexes with structural proteins. These proteins organize the DNA into a compact structure called chromatin. In eukaryotes, this structure involves DNA binding to a complex of small basic proteins called histoneswhile in prokaryotes multiple types of proteins are involved.

These non-specific interactions are formed through basic residues in the histones, making ionic bonds to the acidic sugar-phosphate backbone of the DNA, and are thus largely independent of the base sequence. In humans, replication protein A is the best-understood member of this family and is used in processes where the double helix is separated, including DNA replication, recombination, and DNA repair.

In contrast, other proteins have evolved to bind to particular DNA sequences. The most intensively studied of these are the various transcription factorswhich are proteins that regulate transcription. Each transcription factor binds to one particular set of DNA sequences and activates or inhibits the transcription of genes that have these sequences close to their promoters.

The transcription factors do this in two ways. Firstly, they can bind the RNA polymerase responsible for transcription, either directly or through other mediator proteins; this locates the polymerase at the promoter and allows it to begin transcription. This changes the accessibility of the DNA template to the polymerase. As these DNA targets can occur throughout an organism's genome, changes in the activity of one type of transcription factor can affect thousands of genes.

The specificity of these transcription factors' interactions with DNA come from the proteins making multiple contacts to the edges of the DNA bases, allowing them to "read" the DNA sequence. Most of these base-interactions are made in the major groove, where the bases are most accessible. Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds.

Nucleases that hydrolyse nucleotides from the ends of DNA strands are called exonucleaseswhile endonucleases cut within strands. The most frequently used nucleases in molecular biology are the restriction endonucleaseswhich cut DNA at specific sequences.

In nature, these enzymes protect bacteria against phage infection by digesting the phage DNA when it enters the bacterial cell, acting as part of the restriction modification system. They are also used in DNA repair and genetic recombination. Topoisomerases are enzymes with both nuclease and ligase activity. These proteins change the amount of supercoiling in DNA. Some of these enzymes work by cutting the DNA helix and allowing one section to rotate, thereby reducing its level of supercoiling; the enzyme then seals the DNA break.

Helicases are proteins that are a type of molecular motor. They use the chemical energy in nucleoside triphosphatespredominantly adenosine triphosphate ATPto break hydrogen bonds between bases and unwind the DNA double helix into single strands. Polymerases are enzymes that synthesize polynucleotide chains from nucleoside triphosphates. The sequence of their products is created based on existing polynucleotide chains—which are called templates. Polymerases are classified according to the type of template that they use.

To preserve biological information, it is essential that the sequence of bases in each copy are precisely complementary to the sequence of bases in the template strand. Many DNA polymerases have a proofreading activity. Here, the polymerase recognizes the occasional mistakes in the synthesis reaction by the lack of base pairing between the mismatched nucleotides. They include reverse transcriptasewhich is a viral enzyme involved in the infection of cells by retrovirusesand telomerasewhich is required for the replication of telomeres.

It synthesizes telomeres at the ends of chromosomes. Telomeres prevent fusion of the ends of neighboring chromosomes and protect chromosome Lifeforms (Path 1) - The Future Sound Of London - Cascade / Lifeforms (CD) from damage. As with human DNA-dependent DNA polymerases, RNA polymerase IIthe enzyme that transcribes most of the genes in the human genome, operates as part of a large protein complex with multiple regulatory and accessory subunits.

A DNA helix usually does not interact with other segments of DNA, and in human cells, the different chromosomes even occupy separate areas in the nucleus called " chromosome territories ". Chromosomal crossover is when two DNA helices break, swap a section and then rejoin. Recombination allows chromosomes to exchange genetic information and produces new combinations of genes, which increases the efficiency of natural selection and can be important in the rapid evolution of new proteins.

The most common form of chromosomal crossover is homologous recombinationwhere the two chromosomes involved share very similar sequences. Non-homologous recombination can be damaging to cells, as it can produce chromosomal translocations and genetic abnormalities. The recombination reaction is catalyzed by enzymes known as recombinasessuch as RAD The Holliday junction is Lifeforms (Path 1) - The Future Sound Of London - Cascade / Lifeforms (CD) tetrahedral junction structure that can be moved along the pair of chromosomes, swapping one strand for another.

The recombination reaction is then halted by cleavage of the junction and re-ligation of the released DNA. There are two types of cleavage: east-west cleavage and north—south cleavage. The formation of a Holliday junction during recombination makes it possible for genetic diversity, genes to exchange on chromosomes, and expression of wild-type viral genomes. DNA contains the genetic information that allows all forms of life to function, grow and reproduce. However, it is unclear how long in the 4-billion-year history of life DNA has performed this function, as it has been proposed that the earliest forms of life may have used RNA as their genetic material.

This would occur, since the number of different bases in such an organism is a trade-off between a small number of bases increasing replication accuracy and a large number of bases increasing the catalytic efficiency of ribozymes. Building blocks of DNA adenineguanineand related organic molecules may have been formed extraterrestrially in outer space.

Pyrimidine, like polycyclic aromatic hydrocarbons PAHsthe most carbon-rich chemical found in the universemay have been formed in red giants or in interstellar cosmic dust and gas clouds. In Februaryscientists reported, for the first time, the sequencing of DNA from animal remainsa mammoth in this instance over a million years old, the oldest DNA sequenced to date. Methods have been developed to purify DNA from organisms, such as phenol-chloroform extractionand to manipulate it in the laboratory, such as restriction digests and the polymerase chain reaction.

Modern biology and biochemistry make intensive use of these techniques in recombinant DNA technology. They can be transformed into organisms in the form of plasmids or in the appropriate format, by using a viral vector. Forensic scientists can use DNA in bloodsemenskinsaliva or hair found at a crime scene to identify a matching DNA of an individual, such as a perpetrator.

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